heterozygous b6 sjl slc6a3 tm1 1 cre bkmn j mice  (Jackson Laboratory)

Journal: iScience

Article Title: Genetic dissection of the role of Piga and Pgap2 in the embryonic mouse brain

doi: 10.1016/j.isci.2026.116299

Figure Lengend Snippet: Late onset hypomyelination in Olig2 ; Piga mutant brains (A) Percent genotype ratios of wildtype, Olig2 Cre/wt ; Piga flox/X female and Olig2 Cre/wt ; Piga flox/Y male survival to P21 ( n = 96, Chi-square p = 0.609). Immunostaining of MBP (B–D), CONTACTIN1 (E–G), SOX10 (H–J) and PDGFRα (K–M) in wild type (B, E, H, and K) Olig2 Cre/wt ; Piga flox/X female (C, F, I, and L) and Olig2 Cre/wt ; Piga flox/Y male (D, G, J, and M) animals. MBP and CONTACTIN1 data are from P96 (scale bar, 500 μm) while SOX10 and PDGFRα are from P21 (scale bar, 25 μm) ( n = 4 animals per genotype). Quantification of SOX10 (N, n = 3 animals per genotype, ANOVA p = 0.170), PGDFRα (O, n = 4, ANOVA p = 0.564) positive cells and the PDGFRα/SOX10 ratio (P, n = 3, ANOVA p = 0.430). Data represent individual values and the mean ± s.e.m. White arrows indicate SOX10 and PDGFRα positive cells.

Article Snippet: Mouse: Olig2 -Cre; B6.129- Olig2 tm1.1(cre)Wdr /J , The Jackson Laboratory , RRID:IMSR_JAX:025567.

Techniques: Mutagenesis, Immunostaining

Journal: bioRxiv

Article Title: Endotoxemia and TLR4 via tissue resident macrophages triggers anemia in mouse model of colitis

doi: 10.64898/2026.03.16.712224

Figure Lengend Snippet: A) Flow cytometry histograms of TLR4 expression on CD11b + CSF1R + Ly6G - peritoneal Mφ in Siglec1 Cre :Tlr4 fl/fl mice (red) and control Siglec1 Cre :Tlr4 WT/WT mice (black). B) Frequency of TLR4 + cells gated on CD11b + CSF1R + Ly6G - peritoneal Mφ. Data are from one experiment. Statistical analyses were performed by Mann–Whitney test. Each dot is a separate mouse. Bars represent mean ± SD (n=5/ Siglec1 Cre :Tlr4 WT/WT and n=4/ Siglec1 Cre :Tlr4 fl/fl group). C) RBC counts, D) HB concentrations and E) HCT in the blood after 7 days treatment with DSS or plain water. Data are pooled from 4 independent experiments (n=14/ Siglec1 Cre :Tlr4 WT/WT + water, n=17/ Siglec1 Cre :Tlr4 WT/WT +DSS, n=16/ Siglec1 Cre :Tlr4 fl/fl + water and n=18/ Siglec1 Cre :Tlr4 fl/fl + DSS group). F) Flow cytometry histograms showing lower TZO + Ter119 + blood reticulocyte frequency in Siglec1 Cre :Tlr4 fl/fl mice compared to control Siglec1 Cre :Tlr4 WT/WT mice at day 7 of DSS treatment. G) Quantification of reticulocytes in the blood at day 7 of DSS or water treatment in Siglec1 Cre :Tlr4 fl/fl mice (red) and control Siglec1 Cre :Tlr4 WT/WT mice (black). Data are pooled from 3 independent experiments (n=11/ Siglec1 Cre :Tlr4 WT/WT + water, n=11/ Siglec1 Cre :Tlr4 WT/WT + DSS, n=14/ Siglec1 Cre :Tlr4 fl/fl ± DSS group). H) Photographs of mouse spleens and I) spleen weights. J) Flow cytometry plots showing changes in numbers of proerythroblast and erythroblast subsets (I : Pro-E, II : Baso-E, III ; Poly-E, IVa : Ortho-E) in DSS treated Siglec1 Cre :Tlr4 WT/WT and Siglec1 Cre :Tlr4 fl/fl mice. K) Number of proerythroblasts and erythroblasts per spleen quantified by flow cytometry (subsets described in more detail supplemental Figure 4). Data are pooled from 4 independent experiments (n=14/ Siglec1 Cre :Tlr4 WT/WT + water, n=17/ Siglec1 Cre :Tlr4 WT/WT +DSS, n=16/ Siglec1 Cre :Tlr4 fl/fl + water and n=20/ Siglec1 Cre :Tlr4 fl/fl + DSS group). L) Concentration of endotoxin and M) IL-6 in blood. Data are pooled from 2-3 independent experiments (n=5-8/ Siglec1 Cre :Tlr4 WT/WT + water, n=8-13/ Siglec1 Cre :Tlr4 WT/WT +DSS, n=9-14/ Siglec1 Cre :Tlr4 fl/fl + water and n=9-12/ Siglec1 Cre :Tlr4 fl/fl + DSS group). Each dot is a separate mouse. Bars represent mean ± SD. Statistical analyses were performed by One-Way ANOVA with Sidak’s multiple comparison test.

Article Snippet: B6(Cg)- Tlr4 tm1.1Karp /J (RRID:IMSR_JAX:024872; Tlr4 fl/fl ) and B6.Cg- Gt(ROSA)26Sor tm6 (CAG–ZsGreen1) Hze /J ( R26 ZsG (RRID:IMSR_JAX:007906)) mice 21 were purchased from Jackson laboratory (Bar Harbor, Maine, USA).

Techniques: Flow Cytometry, Expressing, Control, MANN-WHITNEY, Concentration Assay, Comparison

Journal: bioRxiv

Article Title: Endotoxemia and TLR4 via tissue resident macrophages triggers anemia in mouse model of colitis

doi: 10.64898/2026.03.16.712224

Figure Lengend Snippet: A) Representative immunohistofluorescence images of colon sections from Siglec1 Cre :R26 ZsG reporter mice. From left to right fluorescence of ZsGreen reporter, IBA1 (red), CD169 (yellow) tissue Mφ antigens and overlayed signals (n=3). B) Kinetics of relative mouse body weight changes through DSS treatment in Siglec1 Cre :Tlr4 fl/fl (red) and Siglec1 Cre :Tlr4 WT/WT mice (black). Data are pooled from 4 independent experiments (n=13/ Siglec1 Cre :Tlr4 WT/WT +DSS, n=20/ Siglec1 Cre :Tlr4 fl/fl + DSS group). Statistical analyses were performed by Two-Way ANOVA with Sidak’s multiple comparison test. Each dot represents mean ± SD. C) Photograph of colon length of Siglec1 Cre :Tlr4 WT/WT and Siglec1 Cre :Tlr4 fl/fl mice treated for 7 days with DSS (D) or water (W). D) Colon weights and lengths were recorded at the end point and weight over length ratio calculated for each mouse. Data are pooled from 4 independent experiments (n=14/ Siglec1 Cre :Tlr4 WT/WT + water, n=17/ Siglec1 Cre :Tlr4 WT/WT +DSS, n=16/ Siglec1 Cre :Tlr4 fl/fl + water and n=20/ Siglec1 Cre :Tlr4 fl/fl + DSS group). Statistical analyses were performed by One Way ANOVA with Sidak’s multiple comparison test. Each dot is a separate mouse. Bars represent mean ± SD. E) Representative hematoxylin and eosin staining of mouse colon sections and F) quantification of histology scores of distal colon (DC) in Siglec1 Cre :Tlr4 WT/WT and Siglec1 Cre :Tlr4 fl/fl mice treated for 7 days with water or DSS. Data are pooled from 3 independent experiments (n=9/ Siglec1 Cre :Tlr4 WT/WT + water, n=13/ Siglec1 Cre :Tlr4 WT/WT +DSS, n=12/ Siglec1 Cre :Tlr4 fl/fl + water and n=16/ Siglec1 Cre :Tlr4 fl/fl + DSS group). Statistical analyses were performed by One-Way ANOVA with Sidak’s multiple comparison test. G) IL-6 concentration in culture supernatants of colon explants isolated from Siglec1 Cre :Tlr4 WT/WT (black) and Siglec1 Cre :Tlr4 fl/fl (red) mice treated for 7 days with water or 3% DSS. Data are from two independent experiments (n=7-9/group). Statistical significances were calculated by One-Way ANOVA with Sidak’s multiple comparison test. Each dot represents result from colon explant from a separate mouse. Bars represent mean ± SD.

Article Snippet: B6(Cg)- Tlr4 tm1.1Karp /J (RRID:IMSR_JAX:024872; Tlr4 fl/fl ) and B6.Cg- Gt(ROSA)26Sor tm6 (CAG–ZsGreen1) Hze /J ( R26 ZsG (RRID:IMSR_JAX:007906)) mice 21 were purchased from Jackson laboratory (Bar Harbor, Maine, USA).

Techniques: Immunohistofluorescence, Fluorescence, Comparison, Staining, Concentration Assay, Isolation